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Category: Instrumentation

Resource to Compare Analyzers

From Stephanie Morgan:  We are comparing analyzers that perform factor assays, platelet studies, thrombosis testing, and other specialty testing while remaining cost efficient. Where would be the best resource to compare analyzers in each category? Thank you.

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More On Optical Versus Mechanical Clot Detection

In follow-up to Lisa Bakken’s June 19, 2014 question, here is Cunningham, MT, Olson JD, Chandler WL, el al. External quality assurance of fibrinogen assays using normal plasma; results of the 2008 College of American Pathologists Proficiency Testing Program
in Coagulation, Arch Pathol Lab Med 2012;136:789–95. This report was provided by a colleague, and addresses the dispersion of CAP survey fibrinogen results among various instrument/reagent combinations.

Click here for the article: Cunningham et al 2012.

Coag Tests for a Community Hospital

From Meg Harlin, Peninsula Regional Medical Center in Maryland: Our Laboratory will be bringing in new coagulation analyzers from Instrumentation Laboratories, Inc.; TOPS 300, in February, 2014. We currently perform prothrombin time and international normalized ratio (PT/ INR), partial thromboplastin time (APTT, PTT), fibrinogen, and D-dimer. A Few years ago we stopped doing bleeding times, mixing studies and circulating inhibitors when the lab became a lean core. We are a trauma center, and also do a lot of cardiac and orthopedics. We do have the VerifyNow for platelet function for anti-P2Y12 agents and aspirin. Do you think it would be beneficial if we started thrombin times (TT) and anti-Xa’s at this point of starting up new instrumentation? Thank you for your time with my question, I look forward to hearing from you.

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FVIII Inhibitor Assays

From Saravanan Vinayagam: We are trying to set up the factor VIII inhibitor assay on our ACL TOP analysers. Has anyone got an SOP or previous experience in validating inhibitor assay in an automated analysers? Thank you.

Hello, Saravanan Vinayagam, and thank you for your post. I’m hoping that an IL technical representative will see this and offer some assistance. Geo.

Correlating ACT from the i-STAT and Hemochron

A message from Sue Allen at Baptist Hospital: George, a while back you posted a question to the masses about the correlation between the Abbott i-STAT ACT versus the ITC Hemochron 401 and the Hemochron Jr. and I was wondering if you ever received any responses and have any further information on the subject. We are currently doing the same conversion and needless to say, the methods do not directly correlate. They do quite well at the lower ranges but at the 300–350 sec levels they seem to differ by as much as 100 seconds.Thank you in advance for any light you can shed on the subject.

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Participant Poll: Who Uses TEG?

From Kim Kinney at Indiana University Medical Center (IU Health): Hi George. We are being asked to make the thromboelastograph (TEG) available in our special coag lab for clinical use. I am curious to poll others regarding the use of TEG, who is running the test, who is interpreting the results, etc. We have had the TEG available for several research studies here at IU Health but the testing was performed by a research lab. Thanks for the help, Kim

Hi, Kim, it is great to hear from you again, and I hope we get some feedback! I rarely see TEGs in clinical labs, they seem to live mostly in operating suites where they are used to monitor heparin or thrombolytic therapy. Geo.

Dabigatran and the iSTAT

From “SwimRose:”

Hi George, We recently had a case in our ER of a stroke patient on Pradaxa giving an INR of 2.0 with ISTAT, but the main lab instrument, an IL ACL, gave 1.1. Please comment.

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Reprise: Validating POC INRs

Back to our April 24 anticoagulation clinic manager’s question about when to validate a prolonged point of care (POC) prothrombin time with international normalized ratio (PT/INR). George spoke with representatives of Roche Diagnostics, distributor of Coag-U-Chek instruments, while attending the May 3–5 Thrombosis and Hemostasis Summit of North America meeting in Chicago. While Roche contends this is a local decision and makes no firm recommendation, their managers assert that most clinic operators choose 4.0 as the INR above which they send the patient for a follow-up venous PT/INR.

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