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Category: Quick Question Archive

QQ Results: Overfilled Coag Tubes

From our summer, 2014 Quick Question, What is your policy for managing overfilled coagulation specimen tubes?

a. We reject overfilled tubes; results are likely to be invalid. 116 votes, 69%
b. We accept overfilled tubes; overfilling doesn’t affect results. 28 votes, 17%
c. We never see overfilled tubes. 24 votes, 14%

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Quick Question: PTT Mixing Studies

The results of our January-February 2014 Quick Question:

Does your laboratory perform follow-up partial thromboplastin time (PTT) mixing studies 24/7?

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Quick Question: Measuring Dabigatran

This question was a reprise of an earlier question and attracted relatively few votes, however it added “In-house dilute thrombin time,” which turns out to be used by several laboratories.
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In-house Dilute TT

Dear George, I greatly appreciate your website and congratulate you on have such a informative and open educational forum. I was wondering if it was possible to add “in-house dilute thrombin time” to the current Quick Question list? Thank you for your consideration. Sincerely, Glen Holloway.

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QQ Summary: Heparin Monitoring History

Results of our September, 2012 Quick Question:

Historically, what assay was used to monitor heparin?

a. Bleeding time: 14 (17%)
b. Recalcification time: 7 (9%)
c. Lee-White clotting time: 51 (63%)
d. Thromboplastin generation time: 9 (11%)

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New QQ: History

Our August and September Quick Question, posted Monday, August 27, is mostly for fun, and will appeal most to our more “seasoned” coagulationists. No wrong answer, just some more right than others! Please indicate the assay most often used before the partial thromboplastin time (PTT) for monitoring heparin.

Quick Question Summary: VWF Activity

Here is a summary of our July, 2012 Quick Question:

How do you assay for von Willebrand factor (VWF) activity?

a. VWF ristocetin cofactor (VWF:RCo) by aggregometry: 21 (34%)
b. Automated VWF ristocetin cofactor (VWFRCo): 21 (34%)
c. VWF activity immunoassay (VWF:Act): 15 (25%)
d. Collagen binding assay (CBA): 4 (7%)

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New Quick Question: VWF Activity Assays

There’s a definitive article in the June, 2012 JTH that describes the three methods currently available to assay von Willebrand factor activity: ristocetin cofactor (VWF:RCo), collagen binding (VWF:CB), and a monoclonal antibody-based immunoassay (VWF:Act). See Favaloro EJ, Bonar R, Chapman K, Meiring M, Funk (Adcock) D. Differential sensitivity of von Willebrand factor (VWF) ‘activity’ assays to large and small VWF molecular weight forms: a cross-laboratory study comparing ristocetin cofactor, collagen-binding and mAb-based assays. J Thromb Haemost 2012;10: 1043–54. Based on this article, this month’s Quick Question asks which VWF activity assay method you use in your laboratory. Please check out the question at the right and select your answer.

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